Generation of a human iPSC line GIBHi002-A-2 with a dual-reporter for NKX2-5 using TALENs
Min Zhou, Rui Wei, Yu Jiang, Jian Fu, Yuqing Liu, Bo Yang, Baorong Yu, Yun Lin, Xinru Ran, Wing-Hon Lai, Meng Chu, Yang Hu, Jiayin Yang, Hung-Fat Tse
Journal：Stem Cell Research
IF：4.5
DOI：10.1016/j.scr.2020.102120
PMID：33352530
Published：2020-12-10
research field：分子生物学心血管研究干细胞生物学
Abstract
The human transcription factor NKX2-5 plays an important role in cardiac formation and development, and thus it can be used for isolation of cardiomyocytes (CMs) differentiated from human pluripotent stem cells (hPSCs). Here, we knocked-in enhanced GFP (eGFP) and Pac (a puromycin resistant gene; Puro R ) into the exon 1 coding region of NKX2-5 from a human iPSC line iPSN0003 using TALENs. The generated GIBHi002-A-2 enables us to monitor and optimize cardiac differentiation procedures via the cardiac progenitor cells (CPCs), as well as to isolate iPSC-derived CMs for drug screening.
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