Abstract

A novel bacterium, designated strain SYSU M00256-3T, was isolated from a water sample collected from Pearl River Estuary at Guangzhou, PR China. Its taxonomic position was determined by using a polyphasic approach. Cells of the strain were Gram-staining-negative, motile, aerobic and rod-shaped with peritrichous flagella. It could grow at 15–45 °C, pH 4.0–10.0 and in the presence of 0–7.5 % (w/v) NaCl. The chemotaxonomic features of strain SYSU M00256-3Tincluded ubiquinone-10 (Q-10) as the sole respiratory quinone; phosphatidylcholine, phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid as major polar lipids; summed feature 8 (C18 : 1ω7cand C18 : 1ω6c) as the predominant fatty acids (>70 %). On the basis of 16S rRNA gene sequence analysis, strain SYSU M00256-3Twas most closely related to the type strains ofRoseibium hamelinenseCGMCC 1.12584T(97.7 %) andR. aquaeCGMCC 1.12426T(97.2 %),R. sediminisKCTC 52373T(96.7 %),R. denhamenseCGMCC 1.12583T(96.4 %). The average nucleotide identity (ANI) values betweenR. aestuariiSYSU M00256-3TandR. hamelinenseCGMCC 1.12584T,R. aquaeCGMCC 1.12426T,R. denhamenseCGMCC 1.12583TandR. sediminisKCTC 52373Twere 78.0, 78.2, 77.7 and 78.8, and the dDDH value is 20.0, 20.8, 20.1 and 20.6 correspondingly. Based on the analyses of the phenotypic, genotypic and phylogenetic characteristics, strain SYSU M00256-3Tis characterized to represent a novel species of the genusRoseibium, for which the nameRoseibium aestuariisp. nov. is proposed. The type strain is SYSU M00256-3T(=NBRC 112946T=CGMCC 1.16156T).