产品说明书
FAQ
COA
已发表文献
SARS-CoV-2 Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry. The S Protein of the SARS-CoV-2 virus binds Angiotensin-Converting Enzyme 2 (ACE-2), but with much higher affinity and faster binding kinetics . Polyclonal antibodies to the RBD of the SARS-CoV-2 S1 subunit have been shown to inhibit interaction with the ACE-2 receptor, confirming RBD as an attractive target for vaccinations or antiviral therapy . There is also promising work showing that the RBD may be used to detect presence of neutralizing antibodies present in a patient's bloodstream, consistent with developed immunity after exposure to the SARS-CoV-2 virus. Lastly, it has been demonstrated the S Protein can invade host cells through the CD147/EMMPRIN receptor and mediate membrane fusion.
产品性质
|
别名 |
S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1 |
|
Accession |
QHO60594.1 |
|
表达区间及表达系统 |
Recombinant SARS-COV-2 Spike S1 Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Gln14-Arg683. |
|
分子量 |
Approximately 76.1 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result. |
|
纯度 |
> 95% as determined by SDS-PAGE and HPLC. |
|
活性 |
ELISA Data：Immobilized SARS-COV-2 Spike S1, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 19.4ng/ml determined by ELISA. |
|
内毒素 |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
制剂 |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
|
复溶 |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃保存，一年有效期。
复溶后，-20 °C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。
建议第一次使用时分装冻存，避免反复冻融。
注意事项
- 避免反复冻融。
- 为了您的安全和健康，请穿实验服并戴一次性手套操作。
- 本产品仅作科研用途！
产品数据
HB221025
