Recombinant Human CD38(His Tag)

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产品说明书

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已发表文献

CD38, also known as ADP-ribosyl cyclase, is a Type II integral membrane protein. The enzyme is able to transform NAD(P)+ into three different products with calcium mobilizing ability, cyclic ADP-ribose, NAADP+, and ADP-ribose. CD38 is expressed in B and T lymphocytes, osteoclasts, and in cardiac, pancreatic, liver and kidney cells. Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in many types of cells, including neutrophils and pancreatic beta cells.

 

产品性质

别名(Synonyms

ADPRC 1; T10; CD38; ADP-ribosyl cyclase 1; cADPr hydrolase 1; CD38 molecule

Uniprot No.

P28907-1

表达区间及表达系统(Source

Recombinant Human CD38 Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Val43-Ile300. 

分子量(Molecular Weight)

The protein has a predicted MW of 30.7 kDa. Due to glycosylation, the protein migrates to 45-58 kDa based on Tris-Bis PAGE result.

外观(Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

纯度(Purity

> 95% as determined by SDS-PAGE and HPLC

活性(Activity

1.ELISA DataImmobilized Human CD38, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD38 Antibody, hFc Tag with the EC50 of 28ng/ml determined by ELISA.

2.SPR DataAnti-CD38 Antibody captured on Protein A chip, can bind Human CD38, His Tag with an affinity constant of 0.23μM as determined in a SPR assay.

内毒素(Endotoxin

1.0 EU per 1μg of the protein by the LAL method.

制剂(Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

复溶(Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

冰袋运输-20-80存,一年有效期

复溶后,-20 -80°C,在未开封状态下保存 3-6 个月复溶后,2-8°C 保存 2-7 天。

建议第一次使用时分装存,避免反复冻融。

 

注意事项

1. 避免反复冻融。

2. 为了您的安全和健康,请穿实验服并戴一次性手套操作。

3. 本产品仅作科研用途!

 

产品数据

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HB220624

400-6111-883