CD38, also known as ADP-ribosyl cyclase, is a Type II integral membrane protein. The enzyme is able to transform NAD(P)+ into three different products with calcium mobilizing ability, cyclic ADP-ribose, NAADP+, and ADP-ribose. CD38 is expressed in B and T lymphocytes, osteoclasts, and in cardiac, pancreatic, liver and kidney cells. Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in many types of cells, including neutrophils and pancreatic beta cells.
产品性质
别名(Synonyms) |
ADPRC 1; T10; CD38; ADP-ribosyl cyclase 1; cADPr hydrolase 1; CD38 molecule |
Uniprot No. |
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表达区间及表达系统(Source) |
Recombinant Human CD38 Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Val43-Ile300. |
分子量(Molecular Weight) |
The protein has a predicted MW of 30.7 kDa. Due to glycosylation, the protein migrates to 45-58 kDa based on Tris-Bis PAGE result. |
外观(Physical Appearance) |
Sterile Filtered White lyophilized (freeze-dried) powder. |
纯度(Purity) |
> 95% as determined by SDS-PAGE and HPLC |
活性(Activity) |
1.ELISA Data:Immobilized Human CD38, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD38 Antibody, hFc Tag with the EC50 of 28ng/ml determined by ELISA. 2.SPR Data:Anti-CD38 Antibody captured on Protein A chip, can bind Human CD38, His Tag with an affinity constant of 0.23μM as determined in a SPR assay. |
内毒素(Endotoxin) |
< 1.0 EU per 1μg of the protein by the LAL method. |
制剂(Formulation) |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
复溶(Reconstitution) |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃至-80℃保存,一年有效期。
复溶后,-20 至 -80°C,在未开封状态下保存 3-6 个月。复溶后,2-8°C 保存 2-7 天。
建议第一次使用时分装冻存,避免反复冻融。
注意事项
1. 避免反复冻融。
2. 为了您的安全和健康,请穿实验服并戴一次性手套操作。
3. 本产品仅作科研用途!
产品数据
HB220624