Synonyms

HBV Surface Antigen-preS1 ; L glycoprotein，L-HBsAg，Large S protein，Large surface protein，Major surface antigen.

Source

E.coli-derived HBsAg-preS1

Molecular Weight

Approximately 12.6 kDa, a single non-glycosylated polypeptide chain containing 119 amino acids，with an N-terminal Met

AA Sequence

MGGWSSKPRQ GMGTNLSVPN PLGFFPDHQL DPAFGANSNN PDWDFNPNKD HWPEAHQVGA GAFGPGFTPP HGGLLGWSPQ AQGILTTVPV APPPASTNRQ SGRQPTPISP PLRDSHPQA

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 97% by SDS-PAGE and HPLC analyses.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.

Applications

1. Immunochromatography (capture and conjugate);
2. Preparing monoclonal or polyclonal antibodies for HBsAg-preS1;
3. ELISA.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.