可编程核壳微针贴片以海洋多糖核心壳聚糖壳层打破感染性烧伤创面病理循环

Abstract

Infected burn wounds are difficult to heal due to a self-sustaining cycle of infection, inflammation, oxidative stress, and pain that disrupts orderly tissue regeneration. Herein, we develop a programmable marine polysaccharide-based core-shell microneedle patch (CC-MN) that effectively disrupts this pathological cycle through stage-specific therapeutic regulation. The CC-MN integrates a chito-oligosaccharide (COS) shell and a chondroitin sulfate (CS) core. Its programmability is encoded by the core-shell architecture, enabling rapid COS shell dissolution for early-stage antibacterial intervention, followed by sustained CS core release for prolonged immunomodulatory and pro-regenerative regulation. Specifically, CC-MN achieved antibacterial inhibition rates of 94.0%, 98.8%, and 99.5% against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, respectively. Meanwhile, sustained CS release attenuated inflammation, scavenged excessive reactive oxygen species, reduced pain-associated inflammatory mediators including prostaglandin E2, and promoted macrophage polarization toward a pro-regenerative phenotype. In a Pseudomonas aeruginosa-infected burn wound model, CC-MN treatment reduced wound bacterial burden to ~0.5% of the untreated control and accelerated wound closure to 96.1% by day 16, accompanied by decreased inflammatory cytokine expression, enhanced angiogenesis, and improved collagen remodeling. This work presents a translatable and programmable microneedle platform for comprehensive management of infected burn wounds.