Q：能否做活体动物实验？能否观察胰岛素耐受量？

A：不能做活体实验，人的细胞可以观察胰岛素耐受。

Q：这个有配套的缓冲液吗？

A：没有缓冲液，按照说明书上用稀醋酸或者稀盐酸配置成母液就可以了。

Q：这个做脂肪细胞诱导可以吗？

A：文献中是有以胰岛素诱导脂肪细胞的，不过咱的产品没有客户验证过。这个和胰岛素作用是一样的，理论上是可以的，可以让客户尝试一下。

[1] Chen S, Zhu G, Yang Y, et al. Single-cell analysis reveals transcriptomic remodellings in distinct cell types that contribute to human prostate cancer progression. Nat Cell Biol. 2021;23(1):87-98. doi:10.1038/s41556-020-00613-6(IF:28.824)
[2] Niu B, Liu J, Lv B, et al. Interplay between transforming growth factor-β and Nur77 in dual regulations of inhibitor of differentiation 1 for colonic tumorigenesis. Nat Commun. 2021;12(1):2809. Published 2021 May 14. doi:10.1038/s41467-021-23048-5(IF:14.919)
[3] Li S, Han S, Zhang Q, et al. FUNDC2 promotes liver tumorigenesis by inhibiting MFN1-mediated mitochondrial fusion. Nat Commun. 2022;13(1):3486. Published 2022 Jun 17. doi:10.1038/s41467-022-31187-6(IF:14.919)
[4] Li SL, Yang QQ, Liu XY, et al. Zn-doped Cu₂S quantum dots as new high-efficiency inhibitors against human insulin fibrillation based on specific electrostatic interaction with oligomers. Int J Biol Macromol. 2021;179:161-169. doi:10.1016/j.ijbiomac.2021.03.012(IF:6.953)
[5] Usman M, Li Y, Ke Y, et al. Trappc9 Deficiency Impairs the Plasticity of Stem Cells. Int J Mol Sci. 2022;23(9):4900. Published 2022 Apr 28. doi:10.3390/ijms23094900(IF:5.924)
[6] Yang M, Zhang M, Liu Q, et al. 18β-Glycyrrhetinic acid acts through hepatocyte nuclear factor 4 alpha to modulate lipid and carbohydrate metabolism. Pharmacol Res. 2020;157:104840. doi:10.1016/j.phrs.2020.104840(IF:5.893)
[7] Wang BB, Wang YY, Zhang XY, et al. Bifunctional carbon dots for cell imaging and inhibition of human insulin fibrillation in the whole aggregation process. Int J Biol Macromol. 2020;147:453-462. doi:10.1016/j.ijbiomac.2019.12.267(IF:5.162)
[8] Yang QQ , Jin JC , Xu ZQ , et al. Active site-targeted carbon dots for the inhibition of human insulin fibrillation. J Mater Chem B. 2017;5(10):2010-2018. doi:10.1039/c6tb02823c(IF:4.543)
[9] Zhou Z, Liu F, Wang L, et al. Inflammation has synergistic effect with nicotine in periodontitis by up-regulating the expression of α7 nAChR via phosphorylated GSK-3β. J Cell Mol Med. 2020;24(4):2663-2676. doi:10.1111/jcmm.14986(IF:4.486)
[10] Bi Y, Huang W, Yuan L, et al. HOXA10 improves endometrial receptivity by upregulating E-cadherin†. Biol Reprod. 2022;106(5):992-999. doi:10.1093/biolre/ioac007(IF:4.285)
[11] Shi H, Li XY, Chen Y, et al. Quercetin Induces Apoptosis via Downregulation of Vascular Endothelial Growth Factor/Akt Signaling Pathway in Acute Myeloid Leukemia Cells [published correction appears in Front Pharmacol. 2021 Feb 08;11:640750]. Front Pharmacol. 2020;11:534171. Published 2020 Dec 10. doi:10.3389/fphar.2020.534171(IF:4.225)
[12] Zou Y, Zhao Y, Xiao Z, et al. Comparison of Regenerative Effects of Transplanting Three-Dimensional Longitudinal Scaffold Loaded-Human Mesenchymal Stem Cells and Human Neural Stem Cells on Spinal Cord Completely Transected Rats. ACS Biomater Sci Eng. 2020;6(3):1671-1680. doi:10.1021/acsbiomaterials.9b01790(IF:4.152)
[13] Wang J, Han L, Chen J, Xie Y, Jiang H, Zhu J. Reduction of non-specific toxicity of immunotoxin by intein mediated reconstitution on target cells. Int Immunopharmacol. 2019;66:288-295. doi:10.1016/j.intimp.2018.11.039(IF:3.118)
[14] Chen JX, Li HY, Li TT, et al. Alisol A-24-acetate promotes glucose uptake via activation of AMPK in C2C12 myotubes. BMC Complement Med Ther. 2020;20(1):22. Published 2020 Jan 29. doi:10.1186/s12906-019-2802-3(IF:2.838)
[15] Li D, Wei X, Ma M, et al. FFJ-3 inhibits PKM2 protein expression via the PI3K/Akt signaling pathway and activates the mitochondrial apoptosis signaling pathway in human cancer cells. Oncol Lett. 2017;13(4):2607-2614. doi:10.3892/ol.2017.5761(IF:1.390)