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产品名称: STAT3 luciferase reporter plasmid (STAT3萤火虫荧光素酶报告基因质粒)
  • 产品货号:11503ES03
  • 产品规格:1μg
  • 产品价格:
  • 类别:报告基因相关
   
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    产品名称

    产品编号

    规格

    储存

    价格(元)

    STAT3 luciferase reporter plasmid

    STAT3-Luc荧光素酶报告基因质粒)

    11503ES03

    1μg

    -20 ºC

    1685.00

     

    产品描述

    STAT3-Luc荧光素酶报告质粒(报告基因质粒)(STAT3 luciferase reporter plasmid是翊圣生物自主研发的用于检测STAT3转录活性水平为目的的报告基因。STAT3(signal transducers and activators of transduction-3)报告基因与多种恶性肿瘤的发生、发展和侵袭转移关系密切。

    STAT3报告基因主要用于监控细胞内的STAT3转录活性、肿瘤治疗药物的研发以及基因过表达和RNAi的表型分析等。

    pGMSTAT3-Lu是翊圣生物改造后的哺乳动物真核表达载体,在其多克隆位点插入了多个STAT3结合位点,可以高灵敏度地检测STAT3的激活水平。同时,对载体中预测出的其它转录因子以外的结合位点进行了适当的突变,增加了质粒的转录因子结合特异性。由于质粒体积减小,使得STAT3报告基因更易于转染。

    质粒图谱

    运输与保存方法

    冰袋(wet ice)运输。收到产品后放到-20 ºC保存。

    注意事项

    本质粒未经翊圣生物允许不得用于任何商业用途,也不得移交给订货人实验室以外的任何人或单位。

    为了您的健康,实验操作时请穿实验服和带一次性手套。

    使用说明

    pGMSTAT3-Lu可以采用常规转染方法转染哺乳动物细胞。用荧光素酶检测试剂盒或双荧光素酶检测试剂盒进行检测。

    参考文献

    [1] Kang Y, et al. The radio-sensitizing effect of xanthohumol is mediated by STAT3 and EGFR suppression in doxorubicin-resistant MCF-7 human breast cancer cells. Biochim Biophys Acta. 1830(3):2638-48 (2013).

    [2] Teng J, et al. HBx-dependent activation of Twist mediates STAT3 Control of epithelium-mesenchymal transition of liver cells. J Cell Biochem. 114(5):1097-104 (2013).

    [3] Villa S, et al. The influence of the substitution pattern on the molecular conformation of ureido-1,2,5-oxadiazoles, related to STAT3 inhibitors: chemical behavior and structural investigation. ChemBiodivers. 9(7):1240-53 (2012).

    [4] Han L, et al. MicroRNA-21 expression is regulated by β-catenin/STAT3 pathway and promotes glioma cell invasion by direct targeting RECK. CNS NeurosciTher. 18(7):573-83 (2012).

    [5] Kwon MJ, et al. Protein kinase C-θ promotes Th17 differentiation via upregulation of Stat3. J Immunol. 188(12):5887-97 (2012).

    [6] Seo HS, et al. Apigenin induces apoptosis via extrinsic pathway, inducing p53 and inhibiting STAT3 and NFκB signaling in HER2-overexpressing breast cancer cells. Mol Cell Biochem. 366(1-2):319-34 (2012).

    [7] Lim EJ, et al. Methylsulfonylmethane suppresses breast cancer growth by down-regulating STAT3 and STAT5b pathways. PLoS One. 7(4):e33361 (2012).

    [8] Hahm ER, et al. Sulforaphane inhibits constitutive and interleukin-6-induced activation of signal transducer and activator of transcription 3 in prostate cancer cells. Cancer Prev Res (Phila). 3(4):484-94 (2010).

    [9] Lin L, et al. A novel small molecule, LLL12, inhibits STAT3 phosphorylation and activities and exhibits potent growth-suppressive activity in human cancer cells. Neoplasia. 12(1):39-50 (2010).

    [10] Shin DS, et al. Cryptotanshinone inhibits constitutive signal transducer and activator of transcription 3 function through blocking the dimerization in DU145 prostate cancer cells. Cancer Res. 69(1):193-202 (2009).

    [11] Duan Z, et al. Signal transducers and activators of transcription 3 pathway activation in drug-resistant ovarian cancer. Clin Cancer Res. 12(17):5055-63